Journal: eLife

Article Title: VEGF-C promotes the development of lymphatics in bone and bone loss

doi: 10.7554/eLife.34323

Figure Lengend Snippet:

Article Snippet: Plasma was collected from mice and commercially available ELISA kits were used to measure the circulating levels of VEGF-C (CUSABIO, #CSB-E07361M, Houston, TX) and CTX-1 (CUSABIO, #CSB-E12782M).

Techniques: Blocking Assay, Enzyme-linked Immunosorbent Assay, Isolation, cDNA Synthesis, Software

Journal: Scientific Reports

Article Title: Chondrocytes supplemented to bone graft-containing scaffolds expedite cranial defect repair

doi: 10.1038/s41598-023-46604-z

Figure Lengend Snippet: MicroCT and immunofluorescent (IF) analysis confirm an anabolic response which is unsupported in serum bone markers analysis. Rats were subjected to the experimental procedure described in 2A, sacrificed 12 weeks post-procedure and subjected to microCT (n = 7). ( A ) Depicts representative microCT images. ( B ) Depicts microCT analysis of bone volume (mm 3 ), left-handed panel, and bone volume fraction (BV/TV%), right-handed panel, of BG and cBG. ( C ) Depicts mineral apposition distance (MAD) following Alizarin-complexon and Calcein injections at 2- and 6-weeks post procedure (n = 5). MAD was calculated at 3 areas: (i)–(iii), as specified in Materials and Methods. ( D ) Representative IF images of the three denoted areas (× 20). The arrow point at an area of the osseous tissue within the defect site (possibly bone allograft), which is enlarged in the image underneath. At 12 weeks endpoint, serum was collected and analyzed for ( E ) bone anabolic marker, Procollagen type I N-Terminal Propeptide (P1NP), left-handed panel, and bone catabolic marker, Cross Linked C-telopeptide of type I collagen (CTX-1), right-handed panel, to assess bone turnover. Statistical significance was assessed based on Wilcoxon test ( p < 0.05 denotes as “*”). Panel C illustration was adapted from Carmon et al. 2023 .

Article Snippet: Rat sera was collected at endpoint, diluted 1:10 and sera levels of Cross Linked C-telopeptide of type I collagen (CTX-1, cat# E-EL-R1456, Elabscience, TX), Procollagen type I N-Terminal Propeptide (PINP, cat# MBS2506450, MyBioSource, SD), were measured via ELISA assay kit, in accordance to manufactures protocol.

Techniques: Marker